Human Cyclophilin B antibody

Cat No.
ACB0825

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
k2E2
UniProt No.
P23284
NCBI Accession No.
NP_000933
Alternative names
PPIB, Peptidylprolyl isomerase B, CYPB, SCYLP, CYP-S1: Cyclophilin-B, Cyclophilin B, 26- 216aa, AA553318, AI844835, Cphn 2, Cphn2, CyP 20b, AA408962, CYP S1, MGC14109, MGC2224, peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, PPIase, Rotamase, S cyclophilin

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Cyclophilin B (26-216aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ACB0825 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates of HepG2 (30ug) and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system .
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: K562 cell lysate
Lane 3.: 293T cell lysate
Lane 4.: A549 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: SK-OV-3 cell lysate
Lane 7.: LnCap cell lysate
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Cyclophilin B recombinant protein
Lane 2.: Cyclophilin A recombinant protein
Lane 3.: Cyclophilin D recombinant protein
Lane 4.: Cyclophilin E recombinant protein
Lane 5.: Cyclophilin F recombinant protein
Lane 6.: Cyclophilin G recombinant protein
Lane 7.: Cyclophilin H recombinant protein

Flow cytometry (FACS)

Flow cytometry analysis of Cyclophilin B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.