Human CD44 antibody

Cat No.
ACD0826

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
5C10
UniProt No.
P16070
NCBI Accession No.
NP_001001390
Alternative names
CD44 antigen isoform 3 precursor, CD44 molecule (Indian blood group), MIC4, MDU2, MDU3, CD44 antigen (homing function and Indian blood group system), IN, MC56, Pgp1, CD44R, HCELL, CSPG8, hematopoietic cell E- and L-selectin ligand, chondroitin sulfate proteoglycan 8

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human CD44 (21-145aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS nalysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of CD44 in HeLa cells. The cell was stained with ACD0826 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (200ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD44 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human CD44 protein

Flow cytometry (FACS)

Flow cytometry analysis of CD44 in HeLa cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Flow cytometry analysis of CD44 in PBMC cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.