Human FABP1/L-FABP antibody

Cat No.
AFA0908

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
2G4
UniProt No.
P07148
NCBI Accession No.
NP_001434
Alternative names
Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FABP1 (1-127aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,IHC,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of FABP1 in Hep3B cells. The cell was stained with AFA0908 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: FABP1 Recombinant protein
Lane 2.: HepG2 cell lysate
Lane 3.: Liver tissues lysate

Flow cytometry (FACS)

Flow cytometry analysis of FABP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IHC Comment And Pic

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human FABP1 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.