NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

2B5

UniProt No.

Q9NZC2

NCBI Accession No.

NP_061838

Alternative names

Ttriggering receptor expressed on myeloid cells 2 isoform 1, Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on myeloid cells 2a, TREM-2, Trem2a, Trem2b, Trem2c

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human TREM2 (19-161aa) purified from E. coli

Isotype

IgG3 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of TREM2 in HepG2 cells. The cell was stained with ATR0917 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Magnify

ICC/IF analysis of TREM2 in Raw264.7 cells. The cell was stained with ATR0917 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : 293T cell lysate
Lane 2 : TREM2 19-161aa Transfected 293T cell lysate

Flow cytometry (FACS)

Magnify

Flow cytometry analysis of TREM2 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.