Human Wnt-3a antibody

Cat No.
AWN0924

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
3A6
UniProt No.
P56704
NCBI Accession No.
NP_149122
Alternative names
Wnt3a, Wingless-type MMTV integration site family, member 3A

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Wnt3a (19-352aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, IHC
Usage
The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Wnt3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Wnt3a Transfected 293T cell lysate

IHC Comment And Pic

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Related Publications

    Chen F, et al. Curcumin increased the differentiation rate of neurons in neural stem cells via wnt signaling in vitro study. (J Surg Res. 2014)
    Gong SJ, et al. Upregulation of PP2Ac predicts poor prognosis and contributes to aggressiveness in hepatocellular carcinoma. (Cancer Biol Ther. 2016)

    Pan LH, et al. Oncogenic Wnt3a expression as an estimable prognostic marker for hepatocellular carcinoma. (World J Gastroenterol. 2016)

    Bai D, et al. Attenuation of Focal Adhesion Kinase Reduces Lipopolysaccharide-Induced Inflammation Injury through Inactivation of the Wnt and NF-κB Pathways in A549 Cells. (Biochemistry (Mosc). 2017)

    Meng X, et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. (Am J Transl Res. 2017)

    Xiang T, et al. Oxidored-nitro domain-containing protein 1 promotes liver fibrosis by activating the Wnt/β-catenin signaling pathway in vitro. (Mol Med Rep. 2017)

    Li X, et al. Leptin differentially regulates endochondral ossification in tibial and vertebral epiphyseal plates. Cell Biol Int. 2018

    Xue C, et al. β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. (Biosci Rep. 2018)

    Wang L, et al. Expression of hepatic Wnt5a and its clinicopathological features in patients with hepatocellular carcinoma. (Hepatobiliary Pancreat Dis Int. 2018)

    Liu SQ, et al. Propofol Inhibits Proliferation, Migration, Invasion and Promotes Apoptosis Through Down-Regulating miR-374a in Hepatocarcinoma Cell Lines. (Cell Physiol Biochem. 2018)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.