Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 3A6
- UniProt No.
- P56704
- NCBI Accession No.
- NP_149122
- Alternative names
- Wnt3a, Wingless-type MMTV integration site family, member 3A
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Wnt3a (19-352aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, IHC
- Usage
- The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human
Wnt3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Wnt3a Transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: Wnt3a Transfected 293T cell lysate
IHC Comment And Pic
Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Related Publications
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Chen F, et al. Curcumin increased the differentiation rate of neurons in neural stem cells via wnt signaling in vitro study. (J Surg Res. 2014)
Gong SJ, et al. Upregulation of PP2Ac predicts poor prognosis and contributes to aggressiveness in hepatocellular carcinoma. (Cancer Biol Ther. 2016)
Pan LH, et al. Oncogenic Wnt3a expression as an estimable prognostic marker for hepatocellular carcinoma. (World J Gastroenterol. 2016)
Bai D, et al. Attenuation of Focal Adhesion Kinase Reduces Lipopolysaccharide-Induced Inflammation Injury through Inactivation of the Wnt and NF-κB Pathways in A549 Cells. (Biochemistry (Mosc). 2017)
Meng X, et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. (Am J Transl Res. 2017)
Xiang T, et al. Oxidored-nitro domain-containing protein 1 promotes liver fibrosis by activating the Wnt/β-catenin signaling pathway in vitro. (Mol Med Rep. 2017)
Li X, et al. Leptin differentially regulates endochondral ossification in tibial and vertebral epiphyseal plates. Cell Biol Int. 2018
Xue C, et al. β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. (Biosci Rep. 2018)
Wang L, et al. Expression of hepatic Wnt5a and its clinicopathological features in patients with hepatocellular carcinoma. (Hepatobiliary Pancreat Dis Int. 2018)
Liu SQ, et al. Propofol Inhibits Proliferation, Migration, Invasion and Promotes Apoptosis Through Down-Regulating miR-374a in Hepatocarcinoma Cell Lines. (Cell Physiol Biochem. 2018)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.