Human FKBP52 /FKBP4 antibody

Cat No.
ATGA0142

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT4D3
UniProt No.
Q02790
NCBI Accession No.
NP_002005
Alternative names
FK506 binding protein 4, FKBP52, FKBP59, HBI, Hsp56, p52, PPIase, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, FKBP4, FKBP52 protein, FKBP59, Hsp 56, HSP binding immunophilin, p59 protein, PPIase, Rotamase, Peptidyl prolyl cis trans isomerase, Peptidylprolyl cis trans isomerase, T cell FK506 binding protein 59kD

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FkBP4 (1-459aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,IHC,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of FKBP52 /FKBP4 in HeLa cells. The cell was stained with ATGA0142 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP52 /FKBP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: MCF7 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of FKBP52 /FKBP4 in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IHC Comment And Pic

Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP52 /FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.