Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT4D3
- UniProt No.
- Q02790
- NCBI Accession No.
- NP_002005
- Alternative names
- FK506 binding protein 4, FKBP52, FKBP59, HBI, Hsp56, p52, PPIase, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, FKBP4, FKBP52 protein, FKBP59, Hsp 56, HSP binding immunophilin, p59 protein, PPIase, Rotamase, Peptidyl prolyl cis trans isomerase, Peptidylprolyl cis trans isomerase, T cell FK506 binding protein 59kD
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human FkBP4 (1-459aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, IHC, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of FKBP52 /FKBP4 in HeLa cells. The cell was stained with ATGA0142 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP52 /FKBP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: MCF7 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: MCF7 cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of FKBP52 /FKBP4 in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
IHC Comment And Pic
Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP52 /FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.