Human EPHX1 antibody

Cat No.
ATGA0143

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2E5
UniProt No.
P07099
NCBI Accession No.
NP_000111
Alternative names
epoxide hydrolase 1, epoxide hydrolase 1 microsomal (xenobiotic)

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human EPHX1 (21-455aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of EPHX1 in Hep3B cells. The cell was stained with ATGA0143 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The tissue lysate(30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Liver Tissue lysate
The cell lysates(10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: EPHX1 Transfected 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.