Human GAS7 antibody

Cat No.
ATGA0147

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT4H8
UniProt No.
O60861
NCBI Accession No.
NP_958836
Alternative names
Growth arrest-specific 7 isoform b, KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human GAS7 (1-416aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of GAS7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of GAS7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.