Human TYMS antibody

Cat No.
ATGA0169

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT21S5
UniProt No.
P04818
NCBI Accession No.
NP_001062
Alternative names
HST422, TMS, TS, d TMP synthase, EC 2.1.1.45, MGC88736, Thymidylate synthase, Thymidylate synthetase, Tsase, TYMS, TYMS protein, Tyms thymidylate synthetase

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant TYMS (1-313aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

HeLa cells were stained with monoclonal anti-TYMS antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
ICC/IF analysis of TYMS in HeLa cells. The cell was stained with ATGA0169 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TYMS (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human TYMS protein
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: MCF7 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of TYMS in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.