Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT4C3
- UniProt No.
- Q96SI1
- NCBI Accession No.
- NP_076981
- Alternative names
- BTB/POZ domain-containing protein KCTD15, MGC25497, MGC2628, potassium channel tetramerisation domain containing 15
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human KCTD15 (1-234) purified from E.coli
- Isotype
- IgG3 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Western blot analysis (WB)
The cell lysate of HeLa (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : 293T cell lysate
Lane 2 : KCTD15 Transfected 293T cell lysate
Lane 1 : 293T cell lysate
Lane 2 : KCTD15 Transfected 293T cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.