Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT8G4
- UniProt No.
- P04406
- NCBI Accession No.
- NP_002037
- Alternative names
- Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human GAPDH (1-335aa) purified from E.coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
Immunofluorescence of human HeLa cells stained with Hoechst 3342(Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red
Western blot analysis (WB)
The HeLa cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Anti-human GAPDH antibody(1:1000)
Lane 2.: Anti-human GAPDH antibody(1:2000)
Lane 3.: Anti-human GAPDH antibody(1:4000)
Lane 4.: Anti-human GAPDH antibody(1:6000)
Lane 5.: Anti-human GAPDH antibody(1:8000)
Lane 6.: Anti-human GAPDH antibody(1:10000)
Lane 1.: Anti-human GAPDH antibody(1:1000)
Lane 2.: Anti-human GAPDH antibody(1:2000)
Lane 3.: Anti-human GAPDH antibody(1:4000)
Lane 4.: Anti-human GAPDH antibody(1:6000)
Lane 5.: Anti-human GAPDH antibody(1:8000)
Lane 6.: Anti-human GAPDH antibody(1:10000)
Related Publications
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Migas AA, et al. RUNX1T1/MTG8/ETO gene expression status in human t(8;21)(q22;q22)-positive acute myeloid leukemia cells. (Leuk Res. 2014)
Yu H, et al. Sulfasalazine‑induced ferroptosis in breast cancer cells is reduced by the inhibitory effect of estrogen receptor on the transferrin receptor. (Oncol Rep. 2019)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.