Human GAPDH antibody

Cat No.
ATGA0181

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT8G4
UniProt No.
P04406
NCBI Accession No.
NP_002037
Alternative names
Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, MGC88685, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, G3PDH, OCAS, p38 component, Glyceraldehyde 3 phosphate dehydrogenase, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, OCT1 coactivator in S phase, 38-KD component, OTTHuMP00000174431, OTTHuMP00000174432, wu:fb33a10

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human GAPDH (1-335aa) purified from E.coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Immunofluorescence of human HeLa cells stained with Hoechst 3342(Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red

Western blot analysis (WB)

The HeLa cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Anti-human GAPDH antibody(1:1000)
Lane 2.: Anti-human GAPDH antibody(1:2000)
Lane 3.: Anti-human GAPDH antibody(1:4000)
Lane 4.: Anti-human GAPDH antibody(1:6000)
Lane 5.: Anti-human GAPDH antibody(1:8000)
Lane 6.: Anti-human GAPDH antibody(1:10000)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.