Human ERK1/ERK2 antibody

Cat No.
ATGA0182

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1A4
UniProt No.
P28482
NCBI Accession No.
NP_620407
Alternative names
Mitogen-activated protein kinase 1, ERK, ERK2, ERT1, MAPK2, p38, p40, p41, p41mapk, P42MAPK, PRKM1, PRKM2, Mitogen-activated protein kinase 1 MAP kinase 2, MAPK 2, ERK 2, Extracellular Signal Regulated Kinase 2, MAPK 1.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human MAPK1 (1-360) purified from E.coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 3000. Recommended starting dilution is 1:500
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).

Western blot analysis (WB)

Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.