Human Aurora B antibody

Cat No.
ATGA0185

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2B1
UniProt No.
Q96GD4
NCBI Accession No.
NP_004208
Alternative names
Serine/threonine-protein kinase 12, Aurora-B, AIK2, AIM1, ARK2, AurB, aurkb-sv1, aurkb-sv2, IPL1, STK12, aurkb, AIM1, AuRKB, Aurora 1, STK1, STK5, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, Serine/theronine kinase 12, Serine/threonine protein kinase 12

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Aurora kinase B (1-344aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,FACS
Usage
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Aurora kinase B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human Aurora kinase A
Lane 2.: Recombinant Human Aurora kinase B

Flow cytometry (FACS)

Flow cytometry analysis of Aurora B in LNCap cells. The cell was stained with ATGA0185 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.