Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1A2
- UniProt No.
- P27361
- NCBI Accession No.
- NP_002737.2
- Alternative names
- Mitogen-activated protein kinase 3, ERK1, HS44KDAP, HuMKER1A, P44ERK1, P44MAPK, PRKM3, MAP kinase3, Mitogen-activated protein kinase 3
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human MAPK3 protein (1-137aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot, and Immunofluorescence to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:500
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of MAPK3 in HeLa cells line, stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:500) with goat anti-mouse IgG-Texas Red conjugate (Red)
ICC/IF analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis (WB)
The Recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: MAPK1 recombinant protein
Lane 2.: MAPK3 recombinant protein
Lane 1.: MAPK1 recombinant protein
Lane 2.: MAPK3 recombinant protein
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.