Human Cytokeratin 18 antibody

Cat No.
ATGA0193

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2G3
UniProt No.
P05783
NCBI Accession No.
NP_000215
Alternative names
Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, CK 18, CYK18, Cytokeratin 18, Cytokeratin endo B, K18, Keratin 18, Keratin D, Keratin type I cytoskeletal 18, Kerd

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human KRT18 (79-430aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,IHC
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Cytokeratin 18 in HeLa cells. The cell was stained with ATGA0193 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cytokeratin 18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Lane 3.: MCF7 cell lysate

IHC Comment And Pic

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human Cytokeratin 18 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.