Human Enolase 2/Neuron-specific Enolase antibody

Cat No.
ATGA0273

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT17D10
UniProt No.
P09104
NCBI Accession No.
NP_001966
Alternative names
Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human NSE (1-434aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of NSE in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NSE antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The extracts of mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: NSE Transfected 293T cell lysate
The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: U-87 MG cell lysate
Lane 2.: Jurkat cell lysate
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant protein ENO1
Lane 2.: Recombinant protein NSE (ENO2)
Lane 3.: Recombinant protein ENO3

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.