Human ST13 antibody

Cat No.
ATGA0309

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT5C6
UniProt No.
P50502
NCBI Accession No.
NP_003923
Alternative names
Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human HIP/ST13 (1-369aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,FACS,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ST13 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: SW480 cell lysate
Lane 4.: Jurkat cell lysate
Lane 5.: K562 cell lysate
Lane 6.: LnCap cell lysate
Lane 7.: HeLa cell lysate
Lane 8.: PC3 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of ST13 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Flow cytometry analysis of ST13 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.