Human 14-3-3 gamma antibody

Cat No.
ATGA0312

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT4B9
UniProt No.
P61981
NCBI Accession No.
NP_036611
Alternative names
Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide, YWHAG, 14-3-3 gamma,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, gamma polypeptide 14 3 3 protein gamma, 14 3 3 protein gamma subtype, 14 3 3gamma, 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide, KCIP 1, KCIP1, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human YWHAZ
Lane 2.: Recombinant Human YWHAB
Lane 3.: Recombinant Human YWHAE
Lane 4.: Recombinant Human YWHAH
Lane 5.: Recombinant Human YWHAG
Lane 6.: Recombinant Human SFN
Lane 7.: Recombinant Human YWHAQ
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: A431 cell lysate
Lane 3.: K562 cell lysate
Lane 4.: NIH3T3 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.