Human Fc gamma R1/CD64 antibody

Cat No.
ATGA0324

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT37F7
UniProt No.
P12314
NCBI Accession No.
NP_000557
Alternative names
High affinity immunoglobulin gamma Fc receptor I, CD64, CD64A, FCRI, IGFR1

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FCGR1A (16-292aa) purified from E.coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,FACS,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:2000.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of FCGR1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FCGR1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of FCGR1A in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FCGR1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The recombinant protein (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FCGR1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry (FACS)

Flow cytometry analysis of FCGR1A in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Flow cytometry analysis of FCGR1A in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.