Human PKM2 antibody

Cat No.
ATGA0325

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1B10
UniProt No.
P14618
NCBI Accession No.
NP_002645
Alternative names
pyruvate kinase muscle isoform M2, CTHBP, THBP1, p58, M2-PK, Tumor M2-PK, PK2, PK3, PKM, pyruvate kinase, muscle isoform M2 OIP3, PKM, TCB, OPA interacting protein 3, PKM2, Cytosolic thyroid hormone binding protein, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Thyroid hormone binding protein cytosolic, Tumour Type M2 Pyruvate Kinase, Tumor Type M2 Pyruvate Kinase

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PKM2 (1-531aa) purified from E.coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000 and Immunofluorescence is 1:250.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PKM2 in HeLa cells. The cell was stained with ATGA0325 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of PKM2 in A549 cells. The cell was stained with ATGA0325 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Fig.1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : 293T cell lysate
Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.