Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1B10
- UniProt No.
- P14618
- NCBI Accession No.
- NP_002645
- Alternative names
- Pyruvate kinase muscle isoform M2, Pyruvate kinase M1/2, Pyruvate kinase muscle, Cytosolic thyroid hormone-binding protein, CTHBP, Pyruvate kinase 2/3, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1, THBP1, Opa-interacting protein 3, OIP3, Tumor M2-PK, Tyrosine-protein kinase PKM2, PK2, PK3, PKM2, p58
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human PKM2 (1-531aa) purified from E.coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000 and Immunofluorescence is 1:250.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of PKM2 in HeLa cells. The cell was stained with ATGA0325 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of PKM2 in A549 cells. The cell was stained with ATGA0325 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
Fig.1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : 293T cell lysate
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : 293T cell lysate
Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.