Human Annexin A1 antibody

Cat No.
ATGA0336

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2G5
UniProt No.
P04083
NCBI Accession No.
NP_000691
Alternative names
Annexin-1, Lipocortin I, Calpactin II, Chromobindin-9, p35, ANXA1, ANX1, LPC1, Annexin I, AnnexinA1, AnnexinI, ANX 1, ANX A1, ANXA 1, ANXA1 protein, CalpactinII, Chromobindin 9, Chromobindin9, HGNC:533, Lipocortin 1, Lipocortin I, Lipocortin1, LipocortinI, LPC 1, Phospholipase A2 inhibitory protein,

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Annexin A1 (1-346aa) purified from E.coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The Recombinant protein (10ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human Annexin A1
Lane 2.: HeLa cell lysate
Lane 3.: A549 cell lysate
Lane 4.: A431 cell lysate
The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Anti- Annexin A1 antibody (1:10000) Lane 2.: Anti- Annexin A1 antibody (1:20000)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.