Human KRT20 antibody

Cat No.
ATGA0337

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT10D8
UniProt No.
P35900
NCBI Accession No.
NP_061883
Alternative names
Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20; CK-20; CK20; K20; KRT21

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human KRT20 (1-424aa) purified from E.coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of KRT20 in A549 cells. The cell was stained with ATGA0337 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The Recombinant protein (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human KRT20
Lane 2.: HeLa cell lysate
Lane 3.: A431 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.