Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT10D8
- UniProt No.
- P35900
- NCBI Accession No.
- NP_061883
- Alternative names
- CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human KRT20 (1-424aa) purified from E.coli
- Isotype
- IgG2a kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of KRT20 in A549 cells. The cell was stained with ATGA0337 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The Recombinant protein (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human KRT20
Lane 2.: HeLa cell lysate
Lane 3.: A431 cell lysate
Lane 1.: Recombinant Human KRT20
Lane 2.: HeLa cell lysate
Lane 3.: A431 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.