Human Cytokeratin 14 antibody

Cat No.
ATGA0338

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT13F4
UniProt No.
P02533
NCBI Accession No.
AAH19097
Alternative names
Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human KRT14 (1-472aa) purified from E. coli.
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF, IHC, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of KRT14 in PC3 cells. The cell was stained with ATGA0338 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) and cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human KRT14
Lane 2.: A431 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of KRT14 in A431 cells. The cell was stained with ATGA0338 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

IHC Comment And Pic

Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT14 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.