Human CD14 antibody

Cat No.
ATGA0339

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT87H7
UniProt No.
P08571
NCBI Accession No.
NP_001167576
Alternative names
monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human CD14 (20-349aa) purified from E.coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,FACS
Usage
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD14 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of CD14 in THP-1 cells. The cell was stained with ATGA0339 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Flow cytometry analysis of CD14 in PBMC cells. The cell was stained with ATGA0339 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.