NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT19B4

UniProt No.

P01106

NCBI Accession No.

NP_002458

Alternative names

Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39, c-Myc, MRTL, MYCC

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human C-Myc (410-419aa) with tag purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB

Usage

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The recombinant human SNCA-Myc tag proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000)
Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000)
Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000)

Magnify

The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human SNCA-Myc tag (20ng)
Lane 2. : Recombinant Human Myc-GST tag (50ng)
Lane 3. : Recombinant Human s100A8-Myc tag (50ng)
Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng)