NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT5A7

UniProt No.

P15259

NCBI Accession No.

NP_000281

Alternative names

Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human PGAM2 (1-253aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA,WB,ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of PGAM2 in Hep3B cells. The cell was stained with ATGA0399 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombnant protein (50ng)
Lane 2. : HeLa cell lysate
Lane 3. : HepG2 cell lysate
Lane 4. : 293T cell lysate
Lane 5. : Jurkat cell lysate
Lane 6. : NIH3T3 cell lysate
Lane 7. : A549 cell lysate
Lane 8. : MCF7 cell lysate