Human Peroxiredoxin 3 antibody

Cat No.
ATGA0407

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1F8
UniProt No.
P30048
NCBI Accession No.
NP_006784
Alternative names
Thioredoxin-dependent peroxide reductase mitochondrial, Thioredoxin-dependent peroxide reductase mitochondrial, PRDX3, PRX III, Antioxidant protein 1 (AOP-1), Peroxiredoxin 3, Thioredoxin-dependent peroxide reductase, mitochondrial Antioxidant Protein 1, AOP1, MER5, PRX3, SP22, Peroxiredoxin-3

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Peroxiredoxin3 (63-256aa) purified from E. coli
Isotype
IgG3 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Peroxiredoxin3 in HeLa cells. The cell was stained with ATGA0407 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human PRDX3 protein
Lane 2.: HeLa cell lysate
Lane 3.: HepG2 cell lysate
Lane 4.: TF1 cell lysate
Lane 5.: U87MG cell lysate
Lane 6.: Raji cell lysate
Lane 7.: 293T cell lysate
Lane 8.: Jurkat cell lysate
Lane 9.: MCF7 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.