NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT25H10

UniProt No.

O75874

NCBI Accession No.

NP_005887

Alternative names

Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human IDH1 (1-414aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-A affinity chromatography

Applications

ELISA,WB,ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of IDH1 in Hep3B cells. The cell was stained with ATGA0409 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDH1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NIH3T3 cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : K562 cell lysate
Lane 7 : 293T cell lysate
Lane 8 : MCF7 cell lysate
Lane 9 : LnCaP cell lysate