NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT1G7

UniProt No.

P06733

NCBI Accession No.

NP_001419

Alternative names

Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, PPH, Plasminogen-binding protein, ENO1L1, MBPB1, MPB1, ENO1-IT1, ENO1 intronic transcript 1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Alpha-enolase (1-434aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of ENO1, 2 in HeLa cells. The cell was stained with ATGA0424 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ENO1 (Alpha-enolase) recombinant protein
Lane 2.: ENO2 (Gamma-enolase) recombinant protein
Lane 3.: ENO3 (Beta-enolase) recombinant protein.

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: PC3 cell lysate
Lane 2.: MCF7 cell lysate
Lane 3.: 293T cell lysate
Lane 4.: HeLa cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of ENO1, 2 in LNCap cells. The cell was stained with ATGA0424 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).