Human Enolase 1, 2 antibody

Cat No.
ATGA0424

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1G7
UniProt No.
P06733
NCBI Accession No.
NP_001419
Alternative names
Non-Neuronal Enolase (NNE), Enolase-1, ENO1, MBP-1, MYC promoter-binding protein 1, PPH, tau-crystallin, 2 phospho D glycerate hydro lyase, Alpha enolase, C myc promoter binding protein, EC 4.2.1.11, ENO1L1, Enolase 1, Enolase 1 (alpha), Enolase 1 (alpha) like 1, MBP 1, MBP1, MBPB1, MPB 1, MPB1, PPH, MYC promoter binding protein 1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein,

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Alpha-enolase (1-434aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of ENO1, 2 in HeLa cells. The cell was stained with ATGA0424 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ENO1 (Alpha-enolase) recombinant protein
Lane 2.: ENO2 (Gamma-enolase) recombinant protein
Lane 3.: ENO3 (Beta-enolase) recombinant protein.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: PC3 cell lysate
Lane 2.: MCF7 cell lysate
Lane 3.: 293T cell lysate
Lane 4.: HeLa cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of ENO1, 2 in LNCap cells. The cell was stained with ATGA0424 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.