NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT3B2

UniProt No.

Q16698

NCBI Accession No.

NP_001350

Alternative names

24-dienoyl-CoA reductase mitochondrial, 2,4-dienoyl-CoA reductase, mitochondrial, DECR, NADPH, SDR18C1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human DECR1 (35-335aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA,WB,FACS,ICC/IF

Usage

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of DECR1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DECR1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DECR1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: PC3 cell lysate
Lane 2.: HepG2 cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of DECR1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).