Human Inorganic Pyrophosphatase/PPA1 antibody

Cat No.
ATGA0433

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT4G4
UniProt No.
Q15181
NCBI Accession No.
NP_066952
Alternative names
Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PPA1 (1-289aa) purified from E. coli
Isotype
IgG2b Lambda
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A431 cell lysate
Lane 3. : 293T cell lysate
Lane 4. : HepG2 cell lysate
Lane 5. : U87MG cell lysate
Lane 6. : PC3 cell lysate
Lane 7. : WiDr cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.