Human FKBP14 antibody

Cat No.
ATGA0436

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT18E2
UniProt No.
Q9NWM8
NCBI Accession No.
NP_060416
Alternative names
Peptidyl-prolyl cis-trans isomerase FKBP14, FKBP22, Peptidyl-prolyl cis-trans isomerase FKBP14, 22 kDa FK506 binding protein, FK506 binding protein 14 (22 kDa), FK506 binding protein 14, FKBP 22, FKBP22, Peptidyl prolyl cis trans isomerase, PPIase, Rotamase

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FKBP14 (20-211aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of FKBP14 in HeLa cells. The cell was stained with ATGA0436 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : 293T cell lysate
Lane 2. : FKBP14 Transfected 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.