Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT7E9
- UniProt No.
- P00568
- NCBI Accession No.
- NP_000467
- Alternative names
- Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, Adenylate kinase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human AK1 (1-194aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of AK1 in HeLa cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of AK1 in Hep3B cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of AK1 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: U87-MG cell lysate
Lane 3.: K562 cell lysate
Lane 4.: 293T cell lysate
Lane 5.: HepG2 cell lysate
Lane 6.: A549 cell lysate
Lane 7.: MCF7cell lysate
Lane 8.: SK-OV-3 cell lysate
Lane 9.: PC3 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: U87-MG cell lysate
Lane 3.: K562 cell lysate
Lane 4.: 293T cell lysate
Lane 5.: HepG2 cell lysate
Lane 6.: A549 cell lysate
Lane 7.: MCF7cell lysate
Lane 8.: SK-OV-3 cell lysate
Lane 9.: PC3 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.