Human Thioredoxin Reductase 1/TRXR1 antibody

Cat No.
ATGA0451

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT30F1
UniProt No.
Q16881
NCBI Accession No.
NP_001087240
Alternative names
Thioredoxin reductase 1, GRIM-12, MGC9145, TR, TR1, TRXR1, TXNR, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, GRIM 12, GRIM12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase GRIM 12, Thioredoxin reductase TR1, TR 1, TRXR 1, TXNRD 1.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human TXNRD1 (161-647aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of TXNRD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of TXNRD1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TXNRD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Lane 3.: PC3 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.