Human HO-1/HMOX1/HSP32 antibody

Cat No.
ATGA0454

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1D6
UniProt No.
P09601
NCBI Accession No.
NP_002124
Alternative names
HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Heme oxygenase1 (1-266aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,FACS,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of HMOX in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
ICC/IF analysis of HMOX in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMOX antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Lane 3.: 293T cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: TF-1 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of HMOX in HeLa cells. The cell was stained with ATGA0454 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.