Human ACADS antibody

Cat No.
ATGA0459

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT8B10
UniProt No.
P16219
NCBI Accession No.
AAH25963
Alternative names
Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human ACADS (25-412aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of ACADS in Hep3B cells. The cell was stained with ATGA0459 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of ACADS in A549 cells. The cell was stained with ATGA0459 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : HepG2 cell lysate
Lane 2 : LnCap cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.