NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT5F4

UniProt No.

P15531

NCBI Accession No.

NP_000260

Alternative names

NME/NM23 nucleoside diphosphate kinase 1, Nucleoside diphosphate kinase A, Granzyme A-activated Dnase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NDK A, NDP kinase A, NDPKA, NM23

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human NME1 (1-152aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Magnify

ICC/IF analysis of NME1 in HeLa cells. The cell was stained with ATGA0461 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Magnify

ICC/IF analysis of NME1 in A549 cells. The cell was stained with ATGA0461 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : PC3 cell lysate

Magnify

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant Human NME1 protein
Lane 2 : Recombinant Human NME2 protein
Lane 3 : Recombinant Human NME3 protein
Lane 4 : Recombinant Human NME4 protein