NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT5F4

UniProt No.

P15531

NCBI Accession No.

NP_000260

Alternative names

Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1, Non-metastatic cells 1 AWD, drosophila, homolog of, NDPKA, NM23, NM23-M1, Granzyme A activated DNase, GZMA activated DNase, Metastasis inhibition factor nm23, NDK A, NDP Kinase A, NM23 long variant, included, Nonmetastatic protein 23, NME1-NME2 spliced read-through transcript, included, Non-metastatic cells 1, protein (NM23A) expressed in, Nonmetastatic cells 1, protein expressed in, NME1, Nucleoside diphosphate kinase A, Nonmetastatic protein 23, homolog 1, NM23H1B, included, Tumor metastatic process-associated protein

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human NME1 (1-152aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA,WB,ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Magnify

ICC/IF analysis of NME1 in HeLa cells. The cell was stained with ATGA0461 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Magnify

ICC/IF analysis of NME1 in A549 cells. The cell was stained with ATGA0461 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : PC3 cell lysate

Magnify

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant Human NME1 protein
Lane 2 : Recombinant Human NME2 protein
Lane 3 : Recombinant Human NME3 protein
Lane 4 : Recombinant Human NME4 protein