Human PPID antibody

Cat No.
ATGA0473

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1B8
UniProt No.
Q08752
NCBI Accession No.
NP_005029
Alternative names
Peptidylprolyl isomerase D, PPID, CYPD, CYP-40, Cyclophilin D, 40 kDa peptidyl prolyl cis trans isomerase, 40 kDa peptidyl prolyl cis trans isomerase D, Cyclophilin related protein, Cyclophilin40, CyclophilinD, CYP 40, CYP40, MGC33096.Peptidylprolyl isomerase D, PPIase, PPID, Rotamase

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PPID (1-370aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PPID in Hep3B cells. The cell was stained with ATGA0473 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPID antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: K562 cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: HeLa cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: MCF7 cell lysate
Lane 7.: SK-OV-3 cell lysate
Lane 8.: PC3 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.