NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT3B1

UniProt No.

Q92769

NCBI Accession No.

NP_001518

Alternative names

Histone deacetylase 2, HD2, RPD3, YAF1, KDAC2, Protein deacylase HDAC2

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human HDAC2 (1-488aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of HDAC2 in HeLa cells. The cell was stained with ATGA0510 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human HDAC2

Flow cytometry (FACS)

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Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained with ATGA0510 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).