Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 10T1
- UniProt No.
- Q13568
- NCBI Accession No.
- NP_116032.1
- Alternative names
- interferon regulatory factor 5, irf5
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human IRF-5 (176-240aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with ATGA0518 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with ATGA0518 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: THP-1 cell lysate
Lane 1.: THP-1 cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of IRF5 in THP-1 cells. The cell was stained with ATGA0518 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Related Publications
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Moen SH, et al. Human Toll-like Receptor 8 (TLR8) Is an Important Sensor of Pyogenic Bacteria, and Is Attenuated by Cell Surface TLR Signaling. (Front Immunol. 2019)
Guo J, et al. A promising role of interferon regulatory factor 5 as an early warning biomarker for the development of human non-small cell lung cancer. (Lung Cancer. 2019)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.