Human SET 7/9 antibody

Cat No.
ATGA0524

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
s4E5
UniProt No.
Q8WTS6
NCBI Accession No.
NP_085151
Alternative names
SET domain-containing protein 7, SETD7, KIAA1717, KMT7, SET7, SET9, SET7/9 Histone methyltransferase, EC 2.1.1.43, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, SET domain-containing protein 7, SET7/9, Lysine N-methyltransferase 7, Histone-lysine N-methyltransferase SETD7, SET domain-containing protein 7 FLJ21193, H3 K4 HMTase, H4 lysine-4 specific, Histone H3 K4 methyltransferase, Histone H3 lysine 4 specific methyltransferase, Histone H4-K4 methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone-lysine N-methyltransferase, Lysine methyltransferase, SET 7, SET 7/9, SET 9, SET D7, SET domain containing protein 7, SET domain-containing protein 8,
Additional Information
This product was produced from tissue culture supe

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human SET7/9 (1-366aa) purified from E. coli
Isotype
IgG2b kappa
Purification
Applications
ELISA,WB,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of SET7/9 in HeLa cells. The cell was stained with ATGA0524 (1:100). The secondary antibody (green) was used Alexa Fluor 488.

Western blot analysis (WB)

The Recombinant protein(50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human SET7/9 protein
The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: SET7/9 transfected 293T cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of SET7/9 in Jurkat cells. The cell was stained with ATGA0524 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (darkgray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.