NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

s4E5

UniProt No.

Q8WTS6

NCBI Accession No.

NP_085151

Alternative names

SETD7, SET7, SET9, SET7/9, SET7/9 Histone methyltransferase, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43

Additional Information

This product was produced from tissue culture supernatant.

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human SET7/9 (1-366aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of SET7/9 in HeLa cells. The cell was stained with ATGA0524 (1:100). The secondary antibody (green) was used Alexa Fluor 488.

Western blot analysis (WB)

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The Recombinant protein(50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human SET7/9 protein

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The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: SET7/9 transfected 293T cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of SET7/9 in Jurkat cells. The cell was stained with ATGA0524 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (darkgray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Related Publications

Giannios I, et al. Protein Methyltransferase Inhibition Decreases Endocrine Specification Through the Upregulation of Aldh1b1 Expression. (Stem Cells. 2019)
Si W, et al. SET7/9 promotes multiple malignant processes in breast cancer development via RUNX2 activation and is negatively regulated by TRIM21. (Cell Death Dis. 2020)
Xie H, et al. METTL3/YTHDF2 m6 A axis promotes tumorigenesis by degrading SETD7 and KLF4 mRNAs in bladder cancer. (J Cell Mol Med. 2020)
Cao L, et al. Downregulation of SETD7 promotes migration and invasion of lung cancer cells via JAK2/STAT3 pathway. (Int J Mol Med. 2020)
Jetton TL, et al. SetD7 (Set7/9) is a novel target of PPARγ that promotes the adaptive pancreatic β-cell glycemic response. (J Biol Chem. 2021)
Li X, et al. Associating Preoperative MRI Features and Gene Expression Signatures of Early-stage Hepatocellular Carcinoma Patients using Machine Learning. (J Clin Transl Hepatol. 2022)
Li J, et al. ZKSCAN5 Activates VEGFC Expression by Recruiting SETD7 to Promote the Lymphangiogenesis, Tumour Growth, and Metastasis of Breast Cancer. (Front Oncol. 2022)