Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT11C11
- UniProt No.
- Q8N109
- NCBI Accession No.
- NP_065396
- Alternative names
- CD158F,CD158F1,KIR2DL5,KI2LA_HUMAN
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human KIR/CD158 (22-238aa) purified from E.coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, FACS
- Usage
- The antibody has been tested by ELISA, Western blot analysis and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR/CD158 antibody (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: MCF7 cell lysate
Lane 3.: HepG2 cell lysate
Lane 4.: K562 cell lysate
Lane 1.: 293T cell lysate
Lane 2.: MCF7 cell lysate
Lane 3.: HepG2 cell lysate
Lane 4.: K562 cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of KIR/CD158 in PBMC. The cell was stained with ATGA0525 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.