Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- p6C7
- UniProt No.
- P35813
- NCBI Accession No.
- NP_066283
- Alternative names
- Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2CA, PP2C alpha, PDPC 1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human PP2Calpha/PPM1A (1-382aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA , Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained with ATGA0529 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha/PPM1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : A549 cell lysate
Lane 6 : Raji cell lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Mouse brain tissue lysate
Lane 9 : Mouse liver tissue lysate
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : A549 cell lysate
Lane 6 : Raji cell lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Mouse brain tissue lysate
Lane 9 : Mouse liver tissue lysate
Related Publications
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Kim JS, et al. The interaction of hepatitis B virus X protein and protein phosphatase type 2 Calpha and its effect on IL-6. (Biochem Biophys Res Commun. 2006)
Wang K, et al. TGF-β1/p65/MAT2A pathway regulates liver fibrogenesis via intracellular SAM. (EBioMedicine. 2019)
Wang Z, et al. MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation. (Allergy Asthma Immunol Res. 2020)
Yang J, et al. Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine. (Exp Ther Med. 2020)
Xia YC, et al. lncRNA TSPEAR-AS2, a Novel Prognostic Biomarker, Promotes Oral Squamous Cell Carcinoma Progression by Upregulating PPM1A via Sponging miR-487a-3p. (Dis Markers. 2021)
Feng D,et al. Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage. (Sci Adv. 2022)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.