Human Rac1,2,3 antibody

Cat No.
ATGA0589

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT2G10
UniProt No.
P15153
NCBI Accession No.
NP_002863
Alternative names
Ras-related C3 botulinum toxin substrate 1 isoform Rac1, RAC1, RAC1, p21-Rac1, Ras-like protein TC25, Cell migration-inducing gene 5 protein, Ras-related C3 botulinum toxin substrate 1, Ras-related C3 botulinum toxin substrate 1 isoform Rac1 MGC111543, MIG 5, MIG5, Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, Rac 1, Ras like protein TC25, Ras related C3 botulinum toxin substrate 1, Rho family small GTP binding protein Rac1, TC 25, Ras-related C3 botulinum toxin substrate 2., EN-7, Gx, HSPC022, Ras-related C3 botulinum toxin substrate 2, EN 7, EN7, HSPC 022, p21 Rac 2, p21 Rac2, p21Rac2, RAC 2, Ras related C3 botulinum toxin substrate 2, Ras related C3 botulinum toxin substrate 3, Rho family small GTP binding protein Rac 2, Rho family small GTP binding protein Rac2, Small G protein, as-related C3 botulinum toxin substrate 3, p21-Rac3, Rac1B, RAS related C3 botulinum substrate 3, Ras related C3 botulinum toxin substrate 3, rho family small GTP binding protein Rac3, Rho family small GTP binding protein Rac3, RP23-84C12.18

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human RAC2 (1-189aa) protein purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,FACS
Usage
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human Rac 1 protein
Lane 2.: Recombinant human Rac 2 protein
Lane 3.: Recombinant human Rac 3 protein
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained with ATGA0589 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.