Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- AT82B10
- UniProt No.
- P40337
- NCBI Accession No.
- NP_000542
- Alternative names
- Von Hippel-Lindau tumor suppressor isoform 1, Von Hippel-Lindau tumor suppressor isoform 1,HRCA1, RCA1, VHL1, Von Hippel-Lindau tumor suppressor isoform 1 pVHL, G7 protein, Elongin bindingprotein, HRCA 1, RCA 1, VHL, VHL 1, VHLH, Von Hippel Lindau disease tumor suppressor, vonHippel Lindau syndrome, von Hippel Lindau tumor suppressor, AVH0616
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human VHL (1-154aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of VHL in Hep3B cells. The cell was stained with ATGA0542 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: Raji cell lysate
Lane 4.: Jurkat cell lysate
Lane 5.: A549 cell lysate
Lane 6.: MCF7 cell lysate
Lane 7.: PC3 cell lysate
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: Raji cell lysate
Lane 4.: Jurkat cell lysate
Lane 5.: A549 cell lysate
Lane 6.: MCF7 cell lysate
Lane 7.: PC3 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.