Human SUMO2/3 antibody

Cat No.
ATGA0547

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT10F1
UniProt No.
P61956
NCBI Accession No.
NP_008868
Alternative names
Small ubiquitin-related modifier 2, SuMO-2, SuMO-3, Sentrin-2, HSMT3, SMT3B, SMT3H2, Smallubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S, cerevisiae), HSMT 3,MGC117191, Sentrin 2, Sentrin2, Small ubiquitin like modifier 2, Small ubiquitin related modifier2, SMT 3B, SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 2, Sumo2, Sumo3, ubiquitin likeprotein SMT3B.Small ubiquitin-related modifier 3, SMT3A, SMT3H1, SuMO-3, sumo3, sumo 3
Additional Information
This product was produced from tissue culture supe

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human SUMO2 (1-93aa) purified from E.coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of SUMO2/3 in HeLa cells. The cell was stained with ATGA0547 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: K562 cell lysate
Lane 4.: HL-60 cell lysate
The recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human SUMO1 protein
Lane 2.: Recombinant human SUMO2 protein
Lane 3.: Recombinant human SUMO3 protein

Flow cytometry (FACS)

Flow cytometry analysis of SUMO2/3 in Jurkat cells. The cell was stained with ATGA0547 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.