Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- AT10F1
- UniProt No.
- P61956
- NCBI Accession No.
- NP_008868
- Alternative names
- ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, SuMO3, SuMO2, SMT3H2, SMT3H1, SMT3B, SMT3A, SMT3 suppressor of mif two 3 homolog 2, SMT3 homolog 2, SMT 3B, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), Small ubiquitin related modifier 2, Small ubiquitin like modifier 2, Sentrin2, MGC117191, HSMT3
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human SUMO2 (1-93aa) purified from E.coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of SUMO2/3 in HeLa cells. The cell was stained with ATGA0547 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: K562 cell lysate
Lane 4.: HL-60 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: K562 cell lysate
Lane 4.: HL-60 cell lysate
The recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human SUMO1 protein
Lane 2.: Recombinant human SUMO2 protein
Lane 3.: Recombinant human SUMO3 protein
Lane 1.: Recombinant human SUMO1 protein
Lane 2.: Recombinant human SUMO2 protein
Lane 3.: Recombinant human SUMO3 protein
Flow cytometry (FACS)
Flow cytometry analysis of SUMO2/3 in Jurkat cells. The cell was stained with ATGA0547 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.