His tag antibody

Cat No.
ATGA0544

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT1D6
NCBI Accession No.
Alternative names
6 His epitope tag antibody,Hexa His tag antibody,HHHHHH epitope tag antibody,HHHHHH tag antibody,His tag antibody,Polyhistidine Tag antibody

Product Specification

Host
Mouse
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
6x His synthetic peptide
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1: OTUB2-His tag Transfected 293T cell lysate
Lane 2: GAD-His tag Transfected 293T cell lysate
The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1: Recombinant human TREM1-His tag protein
Lane 2: Recombinant human CD34-His tag protein
Lane 3: Recombinant human ATG3-His tag protein

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.