Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- 3F10
- UniProt No.
- Q14653
- NCBI Accession No.
- NP_001562
- Alternative names
- IRF3, Interferon regulatory factor-3, IRF-3, Interferon regulatory factor 3, IRF 3
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human IRF-3(108-166aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of IRF3 in HeLa cells. The cell was stained with ATGA0560 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow cytometry (FACS)
Flow cytometry analysis of IRF3 in MCF7 cells. The cell was stained
with ATGA0560 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG
(Alexa fluor 488) was used as the secondary antibody. Mouse
monoclonal IgG was used as the isotype control (dark gray), cells
without incubation with primary and secondary antibody was used as
the negative control (light gray).
Flow cytometry analysis of IRF3 in Jurkat cells. The cell was stained
with ATGA0560 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG
(Alexa fluor 488) was used as the secondary antibody. Mouse
monoclonal IgG was used as the isotype control (dark gray), cells
without incubation with primary and secondary antibody was used as
the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.