Human Podoplanin antibody

Cat No.
ATGA0571

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
5E2
UniProt No.
Q86YL7
NCBI Accession No.
NP_006465
Alternative names
TIA2, T1A, T1 ALPHA GENE, T1 alpha, Podoplanin, PDPN, PA2.26 antigen, OTTHuMP00000044504, OTTHuMP00000009640, OTS8, Lung type I cell membrane-associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein isoform a AGGRuS, Lung type I cell membrane associated glycoprotein, hT1alpha2, hT1alpha1, HT1A-1, GP40, Gp38, GP36, Glycoprotein 36 KD
Additional Information
This product was produced from tissue culture supernatant.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PDPN (1-206aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of Podoplanin in HeLa cells. The cell was stained with ATGA0571 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Podoplanin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human Podoplanin protein

Flow cytometry (FACS)

Flow cytometry analysis of Podoplanin in HeLa cells. The cell was stained with ATGA0571 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.