Product Information
- Product Type
- Cell Lysate
Product Specification
- Concentration
- 2mg/ml (determined by BCA assay)
- Formulation
- Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
- Storage
- Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
- Preparation before usage
- Heat lysate to 95C for 5 minutes and rapidly cooling before use.
- General Description
- Jurkat cells are an immortalized line of human T lymphocyte cells take from the peripheral blood of a 14-year-old boy with T cell leukemia, in 1970s. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.
- Quality Control
- Western Blot analyzed by both GAPDH and Beta tubulin antibody
Data
Western blot analysis (WB)
All lanes : Jurkat Whole Cell Lysate (40ug)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.